Part:BBa_K4803015:Design

TVMV^Thr-AI_Stable

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 503
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This Part is expected to suppress the activation of TVMV Protease in the absence of Threonine Protease because it has the highest Cleavage Site stability among the sequences we have evaluated in Dry's simulation by changing the linkers before and after the Thr-Cleavage Site in various ways.

Source

This part is a linker-connected part of TVMV Protease, Thr-Cleavage Site, and Inhibitor of TVMV Protease.

References

Stein, V., & Alexandrov, K. (2014). Protease-based synthetic sensing and signal amplification. Proceedings of the National Academy of Sciences, 111(45), 15934–15939. https://doi.org/10.1073/pnas.1405220111

Mirdita, M., Schütze, K., Moriwaki, Y., Heo, L., Ovchinnikov, S., & Steinegger, M. (2022). Colabfold: Making protein folding accessible to all. Nature Methods, 19(6), 679–682. https://doi.org/10.1038/s41592-022-01488-1